Welcome to the Department of Veterinary Medicine's Core Facility.
The role of the Core Facility is to provide access, training, advice and maintenance of the department's wide range of advanced research instrumentation, from microscopes, flow cytometers and centrifuges to protein purification systems and genetic sequencers, in order to further the department's mission of academic excellence.
Our current instruments are organized into Genomics, Flow Cytometry, Imaging and Proteomics Resources. Additionally, the department's core facility also houses various floor or bench centrifuges and ultracentrifuges equipped with a range of rotor options, as well as a Microm HM550 Cryostat Microtom and a Beckman Coulter LS6500 Liquid Scintillation Counter.
Featured Core Facility Equipment
Illumina MiSeq Sequencing System
The Illumina MiSeq system is designed for a broad range of applications, such as small whole genome (de novo or resequencing), targeted gene, or 16S amplicon metagenomic sequencing applications. For more information, click here.
BD FACS Celesta flow cytometer
The BD FACS Celesta is a robust cell analyzer equipped with 3 laser lines, 12 color filter sets for most commonly used dyes and fluorophores and a High Thoughtput System to automatically read small volume samples from 96-384 well plates for fast and efficient sample analysis. More information on the system is available on our Flow Cytometry page.
ZEISS LSM800 Confocal Microscope
Our LSM800 confocal microscope is equipped with 4 laser lines, 2 GaAsp detectors for fluorescence and a PMT for transmitted light imaging. The instrument is also equipped with a stage-top incubator for live imaging assays. More information on this page.
NanoTemper Monolith NT.115
The Nanotemper Monolith system is designed to measure molecular interactions (Kd) of various types of samples in solution using MicroScale Thermophoresis technology. Check out this instrument's information on our Proteomics Resources page.
Core Facility Resources
The Genomics Resources of the Core Facility include a new Illumina MiSeq Sequencing System enabling a broad range of applications and an ABI 3130xl Genetic Analyzer with a 16 capillary array for increased productivity and sequencing of a vide variety of sample.
The Core Facility's flow cytometry resources comprise a BD FACS Aria II cell sorter with 2 lasers and 7 colors and a BD FACS Celesta cell analyzer with 3 lasers and 12 colors and equipped with a high throughput system, allowing for advanced analysis and fast readout of up to 384 samples per plate.
Imaging resources of the Core Facility include Confocal microscopy, epifluorescence, whole animal imaging and electron microscopy.
The core's biochemistry resources include advanced instrumentation to measure molecule interactions, image and analyze protein expression levels (automated Western blot machine and imager) or patterns in cell cultures (EliSpot Reader) or purify proteins (AKTA systems) and more.
Contact
For more information please contact:
Dr. Matthias Corrotte
Core Facility Director
Email: corrotte@umd.edu
Phone: 301-314-1240
