The Genomics Resources of the department of veterinary medicine include an ABI 3130xl genomic sequencer with 16 capillaries and an Illumina MiSeq System for advanced sequencing projects.
The Illumina MiSeq System is under the management of the Molecular Epidemiology Laboratory headed by Dr Mostafa Ghanem.
Available Instruments
Illumina MiSeq System
The Illumina MiSeq is designed for a broad range of applications and is a powerful genomics tool at the service of the UMD research community.
Applications
The Illumina MiSeq system is designed for a broad range of applications, such as small whole genome (de novo or resequencing), targeted gene, or 16S amplicon metagenomic sequencing applications.
Specifications
Output range: 540 Mb-15Gb
Reads per run: 1-25 million
Max Read Length: 2x300 bp
For more details see: https://www.illumina.com/systems/sequencing-platforms/miseq/specifications.html
User Information
For more information on the MiSeq system, visit:
https://www.illumina.com/systems/sequencing-platforms/miseq.html
For information on possible applications and methods:
https://www.illumina.com/systems/sequencing-platforms/miseq/applications.html
DNA quantification service
The system is also accompanied by a Qubit 4 fluorometer for fast and sensitive DNA quantification. For more information on the Qubit:
https://www.thermofisher.com/us/en/home/industrial/spectroscopy-elemental-isotope-analysis/molecular-spectroscopy/fluorometers/qubit/qubit-fluorometer.html
Contact
If you are interested in using our MiSeq system, please contact Dr. Ghanem in the Molecular Epidemiology Laboratory at the Department of Veterinary Medicine at mghanem@umd.edu.
User Rates
Costs may change and are based off our current price for the reagent kit. Please contact the lab for the most current user rate.
|
Run Description |
UMD |
External |
|
25M reads MiSeq (standard) flow-cell v3 (600-cycle) |
$2,131 |
$2,642 |
|
25M reads MiSeq (standard) flow-cell v3 (150-cycle) |
$1,263 |
$1,856 |
|
15M reads MiSeq (standard) flow-cell v2 (500-cycles) |
$1,638 |
$2,192 |
|
15M reads MiSeq (standard) flow-cell v2 (300-cycles) |
$1,456 |
$2,033 |
|
15M reads MiSeq (standard) flow-cell v2 (50-cycles) |
$1,138 |
$1,738 |
|
Qubit fee, facility-operated, 10 samples minimum |
$5 |
$6 |
ABI 3130xl Genetic Analyzer
The 3130xl genetic analyzer provides users access to automated nucleic acid sequencing and analysis.
Applications
The 3130xl genetic analyzer provides users access to automated nucleic acid sequencing and analysis instrumentation. The Applied Biosystems 3130xl DNA Analyzer is equipped with a 16-capillary array and can run a wide variety of sequencing and fragment analysis applications. The system offers industry-leading performance, as well as sophisticated automation capabilities to save time, reduce costs, and increase productivity.
Specifications
Amperage: 15 A
Capillary Length: 80 cm, 22 cm, 36 cm, 50 cm
For Use With: BigDye™Terminator Kits, dRhodamine Dye Terminator Kit
Number of Capillaries: 16 Capillaries
Sample Volume: > 10µL
Size: Up to 950bp
Software: GeneMapper™ Software
Throughput: 23,040 genotypes/24hr (5-dye DNA sizing), 328,000 bases/24hr (Ultra Rapid Sequencing), 17,280 genotypes/24hr (4-dye DNA sizing), 121,600 bases/24hr (Long Read Sequencing)
Rates
- Genomics Core rates apply to all trained, eligible users. Rates are adjusted each year.
- Users within the Department of VetMed: $10.00/run
- On-campus users not affiliated with the department: N/A
- Users not affiliated with the campus: N/A
User Information
For more details see:
https://tools.thermofisher.com/content/sfs/manuals/4477796.pdf
https://www.thermofisher.com/order/catalog/product/3130XLR
For useful information on the instrument:
https://seqgen.com/instrument-model/dna-analyzer/abi-3130-3130xl/
https://biosci-batzerlab.biology.lsu.edu/Genomics/genomics_3130XL_SACKs.php
Core Facility Contact
For more information please contact:
Dr. Matthias Corrotte
Core Facility Director
Email: corrotte@umd.edu
Phone: 301-314-1240
